Ribitol and flavinogenesis in Eremothecium ashbyii

Studies on the biosynthesis of riboflavin; nitrogen metabolism and flavinogenesis in Eremothecium ashbyii.

Development of specific experimental systems for flavinogenesis using non-growing cell of Eremothecium ashbyii.

Experimental systems using non-growing cell of Eremothecium ashbyii to elucidate the mechanism of flavinogenesis were established in this paper. 1. It was found that vacuum infiltration of purines for 2, 3 and 5min and moderate shaking of non-growing cell brought about a good reproducibility of the stimulatory effects on flavinogenesis by purines and furthermore, a higher flavinogenesis. 2. The...

Guanosine diphosphate glucose and guanosine diphosphate fructose in Eremothecium ashbyii.

Chaykin, S., Law, J., Phillips, A. H., Tchen, T. T. & Bloch, K. (1958). Proc. nat. Acad. Sci., Wash., 44, 998. Cornforth, J. W., Cornforth, R. H., Popjak, G. & Gore, I. Y. (1958). Biochem. J. 69, 146. de Waard, A. (1958). Doctoral Thesis: University of Utrecht, Holland; Investigations on the intermediates in the biosynthesis of fatty acids and cholesterol. Nijmegen: Drukkerij Gebr. Janssen. de ...

Some nutritional requirements for biosynthesis of riboflavin by Eremothecium ashbyii.

Eremothecium ashbyii Guilliermond is a remarkable organism because of its capability of synthesizing relatively large amounts of riboflavin under appropriate conditions. This vitamin is synthesized to a limited extent by a rather wide variety of microorganisms (Pridham, 1952), but those known particularly for their riboflavin producing abilities along with E. a&hbyii are Ashbya gosypi'i (Wicker...

The utilization of the ureide carbon of adenine in the biosynthesis of riboflavin in Eremothecium ashbyii.

There is considerable evidence that a critical reaction in purine synthesis is the closure of the purine ring by the addition of a “formate” group to 4-amino-5-imidazolecarboxamide ribonucleotide (1). Apparently the “formate” is added as an active intermediate, since formate as such has little effect in stimulating purine synthesis. Indeed, there is a demonstrable difference in the utilization ...